NAME

primer_designer.pl -- command-line interface Bio::PrimerDesigner

SYNOPSIS

  ./primer_designer.pl [options] [dna_sequence or file]

Options:

-p|--program   Program (default "primer3")
-n|--number    Number of primer sets to return (default "5")
-b|--binary    Path to binary executable (default "/usr/local/bin")
-m|--method    "local" or "remote" (default "local")
-u|--url       URL of remote primer3/e-PCR system
              (default "aceserver.biotech.ubc.ca/cgi-bin/primer_designer.cgi")
--list-aliases Print alias list to primer3 input options and exit
--list-params  Print a list of primer3/e-PCR input options and exit
-h|--help      Print help and exit

DESCRIPTION

This script tests/demonstrates the Bio::PrimerDesigner interface to primer3 and e-PCR. It can be used with a local installation of the unix binaries or remotely via an HTTP/CGI request.

AUTHOR

Copyright (C) 2003 Sheldon McKay <smckay@bcgsc.bc.ca>, Ken Y. Clark <kclark@cpan.org>.

SEE ALSO

Bio::PrimerDesigner.