NAME
BS_ChromosomeSplicer.pl
VERSION
Version 2.10
DESCRIPTION
This utility inserts features into a chromosome. Custom features should be
defined in config/features.txt. You must define a sequence segment for the
edits;it can be the entire chromosome.
The utility can be run in three modes:
segmentflank - In this mode, a custom feature will be inserted at both
ends of the defined sequence segment. The insert will interrupt any
feature that overlaps the segment edges.
featflank - In this mode, all instances of a feature in the defined sequence
segment will be flanked (3', 5', or both) at a defined distance with an
instance of a custom feature. This insertion will NOT interrupt any
features;if a target feature cannot be flanked without interrupting
another chromosomal feature, you will be informed.
featins - In this mode, a custom feature will be inserted at the five prime
end of the defined sequence segment. You must give the feature a unique
name.
ARGUMENTS
Required arguments:
--CHROMOSOME : The chromosome to be modified
--EDITOR : The person responsible for the edits
--MEMO : Justification for the edits
--ACTION : The action to take.
segmentflank : flank the defined segment with custom features
requires -I
featflank : flank a feature type in the defined segment with
custom features;requires -I, -F, -DIS, -DIR
featins : insert a custom feature at the five prime end of the
defined segment;requires -I, -N
Optional arguments:
--ITERATE : [genome, chromosome (def)] Which version number to increment?
--STARTPOS : The first base eligible for editing, defaults to 1
--STOPPOS : The last base eligible for editing, defaults to chr length
--DESTROY : Whether or not other features should be disrupted by
insertions; default 0 for no
--FEATURE : The feature to be targeted
--INSERT : The custom feature to be inserted;must be an entry in
config/features.txt
--DISTANCE : The distance in base pairs from a feature for an
insertion; required for action featflank
--DIRECTION : [3, 5, 35] Should custom features be inserted 3p, 5p, or
both 3p and 5p of a feature;required for action featflank
--NAME : What to name the inserted feature;required for action featins
--OUTPUT : [html, txt (def)] Format for reporting and output
-h, --help : Display this message