NAME

FAST::Bio::Search::Result::GenericResult - Generic Implementation of FAST::Bio::Search::Result::ResultI interface applicable to most search results.

SYNOPSIS

# typically one gets Results from a SearchIO stream
use FAST::Bio::SearchIO;
my $io = FAST::Bio::SearchIO->new(-format => 'blast',
                            -file   => 't/data/HUMBETGLOA.tblastx');
while( my $result = $io->next_result ) {
    # process all search results within the input stream
    while( my $hit = $result->next_hit ) {  
        # insert code here for hit processing
    }
}

use FAST::Bio::Search::Result::GenericResult;
my @hits = (); # would be a list of FAST::Bio::Search::Hit::HitI objects
# typically these are created from a FAST::Bio::SearchIO stream
my $result = FAST::Bio::Search::Result::GenericResult->new
    ( -query_name        => 'HUMBETGLOA',
      -query_accession   => ''
      -query_description => 'Human haplotype C4 beta-globin gene, complete cds.'
      -query_length      => 3002
      -database_name     => 'ecoli.aa'
      -database_letters  => 4662239,
      -database_entries  => 400,
      -parameters        => { 'e' => '0.001' },
      -statistics        => { 'kappa' => 0.731 },
      -algorithm         => 'blastp',
      -algorithm_version => '2.1.2',
      );

my $id = $result->query_name();

my $desc = $result->query_description();

my $name = $result->database_name();

my $size = $result->database_letters();

my $num_entries = $result->database_entries();

my $gap_ext = $result->get_parameter('e');

my @params = $result->available_parameters;

my $kappa = $result->get_statistic('kappa');

my @statnames = $result->available_statistics;

# TODO: Show how to configure a SearchIO stream so that it generates # GenericResult objects.

DESCRIPTION

This object is an implementation of the FAST::Bio::Search::Result::ResultI interface and provides a generic place to store results from a sequence database search.

Unless you're writing a parser, you won't ever need to create a GenericResult or any other ResultI-implementing object. If you use the SearchIO system, ResultI objects are created automatically from a SearchIO stream which returns FAST::Bio::Search::Result::ResultI objects.

For documentation on what you can do with GenericResult (and other ResultI objects), please see the API documentation in FAST::Bio::Search::Result::ResultI.

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

bioperl-l@bioperl.org                  - General discussion
http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

https://redmine.open-bio.org/projects/bioperl/

AUTHOR - Jason Stajich and Steve Chervitz

Email jason@bioperl.org Email sac@bioperl.org

CONTRIBUTORS

Sendu Bala, bix@sendu.me.uk

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

new

Title   : new
Usage   : my $obj = FAST::Bio::Search::Result::GenericResult->new();
Function: Builds a new FAST::Bio::Search::Result::GenericResult object 
Returns : FAST::Bio::Search::Result::GenericResult
Args    : -query_name        => Name of query Sequence
          -query_accession   => Query accession number (if available)
          -query_description => Description of query sequence
          -query_length      => Length of query sequence
          -database_name     => Name of database
          -database_letters  => Number of residues in database
          -database_entries  => Number of entries in database
          -hits              => array ref of FAST::Bio::Search::Hit::HitI objects
          -parameters        => hash ref of search parameters (key => value)
          -statistics        => hash ref of search statistics (key => value)
          -algorithm         => program name (blastx)
          -algorithm_version   => version of the algorithm (2.1.2)
          -algorithm_reference => literature reference string for this algorithm
          -rid               => value of the BLAST Request ID (eg. RID: ZABJ4EA7014)
          -hit_factory       => FAST::Bio::Factory::ObjectFactoryI capable of making
                                FAST::Bio::Search::Hit::HitI objects

algorithm

Title   : algorithm
Usage   : my $r_type = $hsp->algorithm
Function: Obtain the name of the algorithm used to obtain the Result
Returns : string (e.g., BLASTP)
Args    : [optional] scalar string to set value

algorithm_version

Title   : algorithm_version
Usage   : my $r_version = $hsp->algorithm_version
Function: Obtain the version of the algorithm used to obtain the Result
Returns : string (e.g., 2.1.2)
Args    : [optional] scalar string to set algorithm version value

FAST::Bio::Search::Result::ResultI interface methods

FAST::Bio::Search::Result::ResultI implementation

next_hit

Title   : next_hit
Usage   : while( $hit = $result->next_hit()) { ... }
Function: Returns the next available Hit object, representing potential
          matches between the query and various entities from the database.
Returns : a FAST::Bio::Search::Hit::HitI object or undef if there are no more.
Args    : none

query_name

Title   : query_name
Usage   : $id = $result->query_name();
Function: Get the string identifier of the query used by the
          algorithm that performed the search.
Returns : a string.
Args    : [optional] new string value for query name

query_accession

Title   : query_accession
Usage   : $id = $result->query_accession();
Function: Get the accession (if available) for the query sequence
Returns : a string
Args    : [optional] new string value for accession

query_gi

Title   : query_gi
Usage   : $acc = $hit->query_gi();
Function: Retrieve the NCBI Unique ID (aka the GI #),
          if available, for the query
Returns : a scalar string (empty string if not set)
Args    : none

query_length

Title   : query_length
Usage   : $id = $result->query_length();
Function: Get the length of the query sequence
          used in the search.
Returns : a number
Args    :  [optional] new integer value for query length

query_description

Title   : query_description
Usage   : $id = $result->query_description();
Function: Get the description of the query sequence
          used in the search.
Returns : a string
Args    : [optional] new string for the query description

database_name

Title   : database_name
Usage   : $name = $result->database_name()
Function: Used to obtain the name of the database that the query was searched
          against by the algorithm.
Returns : a scalar string
Args    : [optional] new string for the db name

database_letters

Title   : database_letters
Usage   : $size = $result->database_letters()
Function: Used to obtain the size of database that was searched against.
Returns : a scalar integer (units specific to algorithm, but probably the
          total number of residues in the database, if available) or undef if
          the information was not available to the Processor object.
Args    : [optional] new scalar integer for number of letters in db 

database_entries

Title   : database_entries
Usage   : $num_entries = $result->database_entries()
Function: Used to obtain the number of entries contained in the database.
Returns : a scalar integer representing the number of entities in the database
          or undef if the information was not available.
Args    : [optional] new integer for the number of sequence entries in the db

get_parameter

Title   : get_parameter
Usage   : my $gap_ext = $report->get_parameter('gapext')
Function: Returns the value for a specific parameter used
          when running this report
Returns : string
Args    : name of parameter (string)

available_parameters

Title   : available_parameters
Usage   : my @params = $report->available_paramters
Function: Returns the names of the available parameters
Returns : Return list of available parameters used for this report
Args    : none

get_statistic

Title   : get_statistic
Usage   : my $gap_ext = $report->get_statistic('kappa')
Function: Returns the value for a specific statistic available 
          from this report
Returns : string
Args    : name of statistic (string)

available_statistics

Title   : available_statistics
Usage   : my @statnames = $report->available_statistics
Function: Returns the names of the available statistics
Returns : Return list of available statistics used for this report
Args    : none

FAST::Bio::Search::Report

FAST::Bio::Search::Result::GenericResult specific methods

add_hit

Title   : add_hit
Usage   : $report->add_hit($hit)
Function: Adds a HitI to the stored list of hits
Returns : Number of HitI currently stored
Args    : FAST::Bio::Search::Hit::HitI

hit_factory

Title   : hit_factory
Usage   : $hit->hit_factory($hit_factory)
Function: Get/set the factory used to build HitI objects if necessary.
Returns : FAST::Bio::Factory::ObjectFactoryI
Args    : FAST::Bio::Factory::ObjectFactoryI

rewind

Title   : rewind
Usage   : $result->rewind;
Function: Allow one to reset the Hit iterator to the beginning
          Since this is an in-memory implementation
Returns : none
Args    : none

_nexthitindex

Title   : _nexthitindex
Usage   : private

add_parameter

Title   : add_parameter
Usage   : $report->add_parameter('gapext', 11);
Function: Adds a parameter
Returns : none
Args    : key  - key value name for this parama
          value - value for this parameter

add_statistic

Title   : add_statistic
Usage   : $report->add_statistic('lambda', 2.3);
Function: Adds a parameter
Returns : none
Args    : key  - key value name for this parama
          value - value for this parameter

num_hits

Title   : num_hits
Usage   : my $hitcount= $result->num_hits
Function: returns the number of hits for this query result
Returns : integer
Args    : none

hits

Title   : hits
Usage   : my @hits = $result->hits
Function: Returns the available hits for this Result
Returns : Array of L<FAST::Bio::Search::Hit::HitI> objects
Args    : none

algorithm_reference

Title   : algorithm_reference
Usage   : $obj->algorithm_reference($newval)
Function: 
Returns : string containing literature reference for the algorithm
Args    : newvalue string (optional)
Comments: Formerly named program_reference(), which is still supported
          for backwards compatibility.

program_reference

Title   : program_reference
Usage   : $obj->program_reference()
Function:
Returns : string containing literature reference for the algorithm
Args    : 
Comments: Deprecated - use algorithm_reference() instead.

rid

Title   : rid
Usage   : $obj->rid($newval)
Function:
Returns : value of the BLAST Request ID (eg. RID: ZABJ4EA7014)
Args    : newvalue (optional)
Comments: The default implementation in ResultI returns an empty string
          rather than throwing a NotImplemented exception, since
          the RID may not always be available and is not critical.
          See: (1) http://www.ncbi.nlm.nih.gov/Class/MLACourse/Modules/BLAST/rid.html
               (2) http://www.ncbi.nlm.nih.gov/staff/tao/URLAPI/new/node63.html

no_hits_found

See documentation in FAST::Bio::Search::Result::ResultI::no_hits_found()

set_no_hits_found

See documentation in FAST::Bio::Search::Result::ResultI::set_no_hits_found()

to_string

Title   : to_string
Usage   : print $blast->to_string;
Function: Returns a string representation for the Blast result. 
          Primarily intended for debugging purposes.
Example : see usage
Returns : A string of the form:
          [GenericResult] <analysis_method> query=<name> <description> db=<database
          e.g.:
          [GenericResult] BLASTP query=YEL060C vacuolar protease B, db=PDBUNIQ 
Args    : None