/ 
FAST-1.03
River stage zero No dependents
/ FAST::Bio::SeqIO::swiss

NAME

FAST::Bio::SeqIO::swiss - Swissprot sequence input/output stream

SYNOPSIS

It is probably best not to use this object directly, but rather go through the SeqIO handler system:

use FAST::Bio::SeqIO;

$stream = FAST::Bio::SeqIO->new(-file => $filename,
                          -format => 'swiss');

while ( my $seq = $stream->next_seq() ) {
   # do something with $seq
}

DESCRIPTION

This object can transform FAST::Bio::Seq objects to and from Swiss-Pprot flat file databases.

There is a lot of flexibility here about how to dump things which needs to be documented.

GN (Gene name) line management details

A Uniprot/Swiss-Prot entry holds information on one protein sequence. If that sequence is identical across genes and species, they are all merged into one entry. This creates complex needs for several annotation fields in swiss-prot format.

The latest syntax for GN line is described in the user manual:

http://www.expasy.ch/sprot/userman.html#GN_line

Each of the possibly multiple genes in an entry can have Name, Synonyms (only if there is a name), OrderedLocusNames (names from genomic sequences) and ORFNames (temporary or cosmid names). "Name" here really means "symbol". This complexity is now dealt with the following way:

A new FAST::Bio::AnnotationI class was created in order to store the data in tag-value pairs. This class (FAST::Bio::Annotation::TagTree) is stored in the FAST::Bio::Annotation::Collection object and is accessed like all other annotations. The tag name is 'gene_name'.

There is a single FAST::Bio::Annotation::TagTree per sequence record, which corresponds to the original class that stored this data (FAST::Bio::Annotation::StructuredValue). Depending on how we progress this may change to represent each group of gene names.

For now, to access the gene name tree annotation, one uses the below method:

my ($gene) = $seq->annotation->get_Annotations('gene_name');

If you are only interested in displaying the values, value() returns a string with similar formatting.

There are several ways to get directly at the information you want if you know the element (tag) for the data. For gene names all data is stored with the element-tag pairs:

"element1=tag1, tag2, tag3; element2=tag4, tag5;"

This normally means the element will be 'Name', 'Synonyms', etc. and the gene names the values. Using findval(), you can do the following:

# grab a flattened list of all gene names
my @names = $ann->findval('Name');

# or iterated through the nodes and grab the name for each group
for my $node ($ann->findnode('gene_name')) {
   my @names = $node->findval('Name');
}

The current method for parsing gene name data (and reconstructing gene name output) is very generic. This is somewhat preemptive if, for instance, UniProt decides to update and add another element name to the current ones using the same formatting layout. Under those circumstances, one can iterate through the tag tree in a safe way and retrieve all node data like so.

# retrieve the gene name nodes (groups like names, synonyms, etc).
for my $ann ($seq->annotation->get_Annotations('gene_name')) {

    # each gene name group
    for my $node ($ann->findnode('gene_name')) {
        print "Gene name:\n";

        # each gene name node (tag => value pair)
        for my $n ($node->children) {
            print "\t".$n->element.": ".$n->children."\n";
        }
    }
}

For more uses see FAST::Bio::Annotation::TagTree.

Since Uniprot/Swiss-Prot format have been around for quite some time, the parser is also able to read in the older GN line syntax where genes are separated by AND and various symbols by OR. The first symbol is taken to be the 'Name' and the remaining ones are stored as 'Synonyms'.

Also, for UniProt output we support using other FAST::Bio::AnnotationI, but in this case we only use the stringified version of the annotation. This is to allow for backwards compatibility with code that previously used FAST::Bio::Annotation::SimpleValue or other FAST::Bio::AnnotationI classes.

Optional functions

_show_dna()

(output only) shows the dna or not

_post_sort()

(output only) provides a sorting func which is applied to the FTHelpers before printing

_id_generation_func()

This is function which is called as

print "ID   ", $func($seq), "\n";

To generate the ID line. If it is not there, it generates a sensible ID line using a number of tools.

If you want to output annotations in Swissprot format they need to be stored in a FAST::Bio::Annotation::Collection object which is accessible through the FAST::Bio::SeqI interface method annotation().

The following are the names of the keys which are polled from a FAST::Bio::Annotation::Collection object.

reference   - Should contain FAST::Bio::Annotation::Reference objects
comment     - Should contain FAST::Bio::Annotation::Comment objects
dblink      - Should contain FAST::Bio::Annotation::DBLink objects
gene_name   - Should contain FAST::Bio::Annotation::SimpleValue object

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions, preferably to one of the Bioperl mailing lists. Your participation is much appreciated.

bioperl-l@bioperl.org                  - General discussion
http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:

https://redmine.open-bio.org/projects/bioperl/

AUTHOR - Elia Stupka

Email elia@tll.org.sg

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

next_seq

Title   : next_seq
Usage   : $seq = $stream->next_seq()
Function: returns the next sequence in the stream
Returns : FAST::Bio::Seq object
Args    :

write_seq

Title   : write_seq
Usage   : $stream->write_seq($seq)
Function: writes the $seq object (must be seq) to the stream
Returns : 1 for success and 0 for error
Args    : array of 1 to n FAST::Bio::SeqI objects

_generateCRCTable

Title   : _generateCRCTable
Usage   :
Function:
Example :
Returns :
Args    :

_crc32

Title   : _crc32
Usage   :
Function:
Example :
Returns :
Args    :

_crc64

Title   : _crc64
Usage   :
Function:
Example :
Returns :
Args    :

_print_swissprot_FTHelper

Title   : _print_swissprot_FTHelper
Usage   :
Function:
Example :
Returns :
Args    :

_read_swissprot_References

Title   : _read_swissprot_References
Usage   :
Function: Reads references from swissprot format. Internal function really
Example :
Returns :
Args    :

_read_swissprot_Species

Title   : _read_swissprot_Species
Usage   :
Function: Reads the swissprot Organism species and classification
          lines.
            Able to deal with unconventional species names.
Example : OS Unknown prokaryotic organism
            $genus = undef ; $species = Unknown prokaryotic organism
Returns : A FAST::Bio::Species object
Args    :

_filehandle

Title   : _filehandle
Usage   : $obj->_filehandle($newval)
Function:
Example :
Returns : value of _filehandle
Args    : newvalue (optional)

_read_FTHelper_swissprot

Title   : _read_FTHelper_swissprot
Usage   : _read_FTHelper_swissprot(\$buffer)
Function: reads the next FT key line
Example :
Returns : FAST::Bio::SeqIO::FTHelper object
Args    :

_write_line_swissprot

Title   : _write_line_swissprot
Usage   :
Function: internal function
Example :
Returns :
Args    :

_write_line_swissprot_regex

Title   : _write_line_swissprot_regex
Usage   :
Function: internal function for writing lines of specified
          length, with different first and the next line
          left hand headers and split at specific points in the
          text
Example :
Returns : nothing
Args    : file handle, first header, second header, text-line, regex for line breaks, total line length

_post_sort

Title   : _post_sort
Usage   : $obj->_post_sort($newval)
Function:
Returns : value of _post_sort
Args    : newvalue (optional)

_show_dna

Title   : _show_dna
Usage   : $obj->_show_dna($newval)
Function:
Returns : value of _show_dna
Args    : newvalue (optional)

_id_generation_func

Title   : _id_generation_func
Usage   : $obj->_id_generation_func($newval)
Function:
Returns : value of _id_generation_func
Args    : newvalue (optional)

_ac_generation_func

Title   : _ac_generation_func
Usage   : $obj->_ac_generation_func($newval)
Function:
Returns : value of _ac_generation_func
Args    : newvalue (optional)

_sv_generation_func

Title   : _sv_generation_func
Usage   : $obj->_sv_generation_func($newval)
Function:
Returns : value of _sv_generation_func
Args    : newvalue (optional)

_kw_generation_func

Title   : _kw_generation_func
Usage   : $obj->_kw_generation_func($newval)
Function:
Returns : value of _kw_generation_func
Args    : newvalue (optional)