NAME

Bio::Tools::Analysis::Protein::Scansite - a wrapper around the Scansite server

SYNOPSIS

 use Bio::Tools::Analysis::Protein::Scansite;

 my $seq; # a Bio::PrimarySeqI object

 my $tool = Bio::Tools::Analysis::Protein::Scansite->new
    ( -seq => $seq->primary_seq ); 

 # run Scansite prediction on a sequence
 $tool->run();

 # alternatively you can say
 $tool->seq($seq->primary_seq)->run;

 die "Could not get a result" unless $tool->status =~ /^COMPLETED/;

 print $tool->result;     # print raw prediction to STDOUT

 foreach my $feat ( $tool->result('Bio::SeqFeatureI') ) {

     # do something to SeqFeature
     # e.g. print as GFF
     print $feat->gff_string, "\n";
     # or store within the sequence - if it is a Bio::RichSeqI
     $seq->add_SeqFeature($feat);

}

DESCRIPTION

This class is a wrapper around the Scansite 2.0 server which produces predictions for serine, threonine and tyrosine phosphorylation sites in eukaryotic proteins. At present this is a basic wrapper for the "Scan protein by input sequence" functionality, which takes a sequence and searches for motifs, with the option to select the search stringency. At present, searches for specific phosphorylation sites are not supported; all predicted sites are returned.

Return formats

The Scansite results can be obtained in several formats:

1. By calling

   my $res = $tool->result('');

   $res holds a string of the predicted sites in tabular format.

2. By calling

   my $data_ref = $tool->result('value')

   $data_ref is a reference to an array of hashes. Each element in the array represents a predicted phosphorylation site. The hash keys are the names of the data fields,i.e.,

   'motif'      => 'Casn_Kin1'       # name of kinase
   'percentile' => 0.155             # see Scansite docs
   'position'   => 9                 # position in protein
   'protein'    => 'A1'              # protein id
   'score'      => 0.3696            # see Scansite docs
   'sequence'   => 'ASYFDTASYFSADAT' # sequence surrounding site
   'site'       => 'S9'              # phosphorylated residue
   'zscore'     => '-3.110'          # see Scansite docs

3. By calling

   my @fts = $tool->Result('Bio::SeqFeatureI');

   which returns an array of Bio::SeqFeatureI compliant objects with primary tag value 'Site' and tag names of 'motif', 'score', 'sequence', 'zscore' as above.

See http://scansite.mit.edu/.

This inherits Bio::SimpleAnalysisI which hopefully makes it easier to write wrappers on various services. This class uses a web resource and therefore inherits from Bio::WebAgent.

SEE ALSO

Bio::SimpleAnalysisI, Bio::WebAgent

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.

bioperl-l@bioperl.org                  - General discussion
http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:

https://github.com/bioperl/bioperl-live/issues

AUTHORS

Richard Adams, Richard.Adams@ed.ac.uk,

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

result

Name    : result
Usage   : $job->result (...)
Returns : a result created by running an analysis
Args    : none (but an implementation may choose
          to add arguments for instructions how to process
          the raw result)

The method returns a scalar representing a result of an executed job. If the job was terminated by an error, the result may contain an error message instead of the real data.

This implementation returns differently processed data depending on argument:

undef

Returns the raw ASCII data stream but without HTML tags

'Bio::SeqFeatureI'

The argument string defined the type of bioperl objects returned in an array. The objects are Bio::SeqFeature::Generic.

'parsed'

Returns a reference to an array of hashes containing the data of one phosphorylation site prediction. Key values are:

motif, percentile, position, protein, score, site, zscore, sequence.

stringency

Usage    : $job->stringency(...)
Returns  : The significance stringency of a prediction
Args     : None (retrieves value) or 'High', 'Medium' or 'Low'.
Purpose  : Get/setter of the stringency to be sumitted for analysis.

protein_id

Usage    : $job->protein_id(...)
Returns  : The sequence id of the protein or 'unnamed' if not set. 
Args     : None  
Purpose  : Getter of the seq_id. Returns the display_id of the sequence
           object. 

cpanm Bio::Seq

perl -MCPAN -e shell
install Bio::Seq