NAME
Bio::Tools::Gel - Calculates relative electrophoretic migration distances
SYNOPSIS
use Bio::PrimarySeq;
use Bio::Restriction::Analysis;
use Bio::Tools::Gel;
# get a sequence
my $d = 'AAAAAAAAAGAATTCTTTTTTTTTTTTTTGAATTCGGGGGGGGGGGGGGGGGGGG';
my $seq1 = Bio::Seq->new(-id=>'groundhog day',-seq=>$d);
# cut it with an enzyme
my $ra=Bio::Restriction::Analysis->new(-seq=>$seq1);
@cuts = $ra->fragments('EcoRI'), 3;
# analyse the fragments in a gel
my $gel = Bio::Tools::Gel->new(-seq=>\@cuts,-dilate=>10);
my %bands = $gel->bands;
foreach my $band (sort {$b <=> $a} keys %bands){
print $band,"\t", sprintf("%.1f", $bands{$band}),"\n";
}
#prints:
#20 27.0
#25 26.0
#10 30.0DESCRIPTION
This takes a set of sequences or Bio::Seq objects, and calculates their respective migration distances using: distance = dilation * (4 - log10(length(dna));
Source: Molecular Cloning, a Laboratory Manual. Sambrook, Fritsch, Maniatis. CSHL Press, 1989.
Bio::Tools::Gel currently calculates migration distances based solely on the length of the nucleotide sequence. Secondary or tertiary structure, curvature, and other biophysical attributes of a sequence are currently not considered. Polypeptide migration is currently not supported.
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing listsSupport
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:
https://github.com/bioperl/bioperl-live/issuesAUTHOR - Allen Day
Email allenday@ucla.edu
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
new
Title : new
Usage : my $gel = Bio::Tools::Gel->new(-seq => $sequence,-dilate => 3);
Function: Initializes a new Gel
Returns : Bio::Tools::Gel
Args : -seq => Bio::Seq(s), scalar(s) or list of either/both
(default: none)
-dilate => Expand band migration distances (default: 1)add_band
Title : add_band
Usage : $gel->add_band($seq);
Function: Calls _add_band with a (possibly created) Bio::Seq object.
Returns :
Args : Bio::Seq, scalar sequence, or list of either/both._add_band
Title : _add_band
Usage : $gel->_add_band($seq);
Function: Adds a new band to the gel.
Returns :
Args : Bio::Seq objectdilate
Title : dilate
Usage : $gel->dilate(1);
Function: Sets/retrieves the dilation factor.
Returns : dilation factor
Args : Float or nonebands
Title : bands
Usage : $gel->bands;
Function: Calculates migration distances of sequences.
Returns : hash of (seq_id => distance)
Args : log10
Title : log10
Usage : log10($n);
Function: returns base 10 log of $n.
Returns : float
Args : float