NAME
FAST - FAST Analysis of Sequences Toolbox
VERSION
Version 1.03
SYNOPSIS
The Fast Analysis of Sequences Toolbox (FAST) is a set of UNIX utilities (for example fasgrep, fascut, fashead and fastr) that extends the UNIX toolbox paradigm to bioinformatic sequence records. Modeled after the UNIX textutils (such as grep, cut, head, tr, etc), FAST workflows are designed for "inline" (serial) processing of flatfile biological sequence record databases per-sequence, rather than per-line, through UNIX pipelines. The default data exchange format is multifasta (specifically, a restriction of BioPerl FastA format). FASTQ format is supported. FAST is designed for learnability, interoperability, interface consistency, rapid prototyping, fine-tuned control, and reproducibility. FAST tools expose the power of Perl and BioPerl to users in an easy-to-learn command-line paradigm.
UTILITIES
FAST 1.0x contains the following utilities. Each has its own man page.
- alncut select sites based on site variation and gap-content
- alnpi calculate molecular population genetic statistics
- fascodon tally/annotate codon usage
- fascomp tally/annotate monomer frequencies
- fasconvert convert sequences to or from from fasta format
- fascut select/reorder sequence records based on index lists/ranges
- fasfilter filter sequence records based on numerical values
- fasgrep filter sequence records based on perl regular expressions
- fashead filter leading sequence records
- faslen annotate sequence lengths
- faspaste concatenate sequence records
- fasrc reverse complement nucleotide sequences and alignments
- fassort sort sequence records
- fassub transform sequence records using regex-based substitutions
- fastail filter trailing sequence records
- fastax filter sequence records based on NCBI taxonomy IDs or names
- fastaxsort sort sequence records based on NCBI taxonomy IDs or names
- fastr transform sequence records by character, degap, strict
- fasuniq remove duplicate sequence records from sorted data
- faswc tally sequences and characters
- fasxl translate gapped and ungapped sequences and alignments
- gbfalncut select sites by regex matching of features in a GenBank file
- gbfcut print sequences by regex matching to features in a GenBank file
AUTHORS
David H. Ardell, <dhard at cpan.org>
and members of the Ardell Laboratory and other contributors including: Travis Lawrence Dana Carper Katherine Amrine Kyle Kauffman Claudia Canales
BUGS
Please report any bugs or feature requests to bug-fast at rt.cpan.org
, or through the web interface at http://rt.cpan.org/NoAuth/ReportBug.html?Queue=FAST. I will be notified, and then you'll automatically be notified of progress on your bug as I make changes.
ACKNOWLEDGEMENTS
LICENSE AND COPYRIGHT
Copyright 2015 David H. Ardell.
This program is free software; you can redistribute it and/or modify it under the terms of the the Artistic License (2.0). You may obtain a copy of the full license at:
http://www.perlfoundation.org/artistic_license_2_0
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