NAME
Bio::BioStudio::RestrictionEnzyme
VERSION
Version 2.10
DESCRIPTION
BioStudio object that represents a restriction enzyme - inherits from Bio::GeneDesign::RestrictionEnzyme and adds attributes for feature annotation awareness. This object is heavily used in chromosome segmentation - for other uses the GeneDesign object should be sufficient.
AUTHOR
Sarah Richardson <smrichardson@lbl.gov>
CONSTRUCTORS
new
When this object is created it is also subject to the requirements of the ancestor Bio::GeneDesign::RestrictionEnzyme object. Use the ancestor flag -enzyme with a GeneDesign RestrictionEnzyme object, allowing the ancestral object to "clone" itself to create a new BioStudio RestrictionEnzyme object:
my $newfeat = Bio::BioStudio::RestrictionEnzyme->new(
-enzyme => $BamHI_Bio::GeneDesign::RestrictionEnzyme_object,
-name => "BamHI_56781",
-presence => "potential");
There are two required arguments:
-name the name of the enzyme
-presence (p)otential, (i)ntergenic, (e)xisting, or (a)ppended, the status
of the enzymeThe other arguments are optional:
-eligible whether or not to ignore this enzyme when selecting segmentation
enzymes; usually gets set automatically when the
L<Bio::BioStudio::RestrictionEnzyme::Store> object is created.
May only be undefined (omitted) or "no".
-end The stop coordinate of the enzyme
-feature The L<Bio::DB::SeqFeature> object associated with the enzyme -
usually a gene; sometimes an intergenic region
-featureid The name of the feature associated with the enzyme - this is set
automatically if -feature is used
-overhangs A comma separated list of overhangs that this enzyme can legally
leave. This will be parsed into a hash reference.
-strand [1 or -1] If 1, the object is either oriented 5 prime to 3 prime
on the Watson strand or is symmetric and therefore on both
strands. If -1, the object is on the Crick strand.
-peptide The peptide sequence associated with the enzymes recognition
site. Should only be supplied if the feature object associated
with the enzyme is a gene, mRNA, or CDS object.
-offset This number indicates how far away from the recognition site
the actual cut site is.
-dbid The primary key of this enzyme in the database; usually set by
L<Bio::BioStudio::RestrictionEnzyme::Store> at the time of
database creation
-phang The preferred overhang for use when committing this enzyme to
sequence.FUNCTIONS
line_report
This function outputs the restriction enzyme object in a format that is suitable for quickloading into a MySQL database - this is how the Bio::RestrictionEnzyme::Store database becomes populated.
ACCESSORS
name
The name of the enzyme; current BioStudio custom is that the name of an enzyme is its id (ie, the name of the protein), underscore, start coordinate. That is, BamHI_54671, or SacII_4689. But this is entirely arbitrary and will not impact processing.
presence
Enzymes can be any one of the following:
(p)otential : can be introduced into exonic sequence without changing protein
sequence - but does not exist now
(i)ntergenic : exists in a non-exonic region. Most BioStudio algorithms do not
support editing such an enzyme.
(e)xisting : or exonic, this site is in a gene region and can be edited or
removed.
(a)ppended : this site doesn't exist but will be appended to the sequence
end
Bio::GeneDesign::RestrictionEnzyme objects have a start attribute, but not an end attribute.
eligible
Should this enzyme be considered for landmark status or not? This flag is usually set when the Bio::BioStudio::RestrictionEnzyme::Store database is created and populated. Enzymes whose presence is potential and whose eligibility is null are usually deleted rather than marked ineligible.
The only argument this accessor accepts is a string value of "no".
featureid
The id of the feature associated with the enzyme; this is a string and is useful when pulling objects out of the MySQL database, which currently doesn't store an actual Bio::DB::SeqFeature object, but only its id. Given the id, the feature can be looked up from a Bio::DB::SeqFeature::Store object.
overhangs
A hash reference where the keys are possible overhangs that may be left by the enzyme should it be edite into sequence.
strand
Is the recognition site on the Watson (1) or Crick (-1) strand?
If 1, the object is either oriented 5 prime to 3 prime on the Watson strand or is symmetric and therefore on both strands. If -1, the object is on the Crick strand.
peptide
IF the enzyme occurs in a gene, what peptide sequence (in the first frame of translation) covers the recognition site? Should only be defined if the associated feature is a CDS.
offset
How far away the overhang is from the recognition site, in bases (if there is an overhang).
movers
If this enzyme is introduced or edited into sequence, which other restriction enzyme recognition sites must be removed to make it a unique landmark?
Takes and returns an array reference, where each entry in the array is the name of a BioStudio restriction enzyme object.
creates
If this enzyme is introduced or edited into sequence, which other restriction enzyme recognition sites will it create?
Takes and returns an array reference, where each entry in the array is the id of a GeneDesign restriction enzyme object.
dbid
The entry in the primary key column of the MySQL database underlying the Bio::BioStudio::RestrictionEnzyme::Store object; this is usually used during database creation and culling and is unneccesary otherwise.
phang
If this enzyme is to be introduced or edited into sequence, which of its possible overhangs should be used? The argument must be a key that exists in the overhangs hash reference.
COPYRIGHT AND LICENSE
Copyright (c) 2014, BioStudio developers All rights reserved.
Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met:
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* The names of Johns Hopkins, the Joint Genome Institute, the Lawrence Berkeley National Laboratory, the Department of Energy, and the BioStudio developers may not be used to endorse or promote products derived from this software without specific prior written permission.
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