NAME

Bio::Tools::Run::StandAloneBlastPlus::BlastMethods - Provides BLAST methods to StandAloneBlastPlus

SYNOPSIS

# create a factory:
$fac = Bio::Tools::Run::StandAloneBlastPlus->new(
   -db_name => 'testdb'
);
# get your results
$result = $fac->blastn( -query => 'query_seqs.fas',
                        -outfile => 'query.bls',
                        -method_args => [ '-num_alignments' => 10 ] );

$result = $fac->tblastx( -query => $an_alignment_object,
                         -outfile => 'query.bls',
                         -outformat => 7 );
# do a bl2seq
$fac->bl2seq( -method => 'blastp',
              -query => $seq_object_1,
              -subject => $seq_object_2 );

DESCRIPTION

This module provides the BLAST methods (blastn, blastp, psiblast, etc.) to the Bio::Tools::Run::StandAloneBlastPlus object.

USAGE

This POD describes the use of BLAST methods against a Bio::Tools::Run::StandAloneBlastPlus factory object. The object itself has extensive facilities for creating, formatting, and masking BLAST databases; please refer to Bio::Tools::Run::StandAloneBlastPlus POD for these details.

Given a StandAloneBlastPlus factory, such as

$fac = Bio::Tools::Run::StandAloneBlastPlus->new(
   -db_name => 'testdb'
);

you can run the desired BLAST method directly from the factory object, against the database currently attached to the factory (in the example, testdb). -query is a required argument:

$result = $fac->blastn( -query => 'query_seqs.fas' );

Here, $result is a Bio::Search::Result::BlastResult object.

Other details:

• The blast output file can be named explicitly:

  $result = $fac->blastn( -query => 'query_seqs.fas',
                          -outfile => 'query.bls' );

• The output format can be specified:

  $result = $fac->blastn( -query => 'query_seqs.fas',
                          -outfile => 'query.bls',
                          -outformat => 7 ); #tabular

• Additional arguments to the method can be specified:

  $result = $fac->blastn( -query => 'query_seqs.fas',
                          -outfile => 'query.bls',
                          -method_args => [ '-num_alignments' => 10 ,
                                            '-evalue' => 100 ]);

• HTML output can be created using this workaround:

  $result = $fac->blastn( -query => 'query_seqs.fas',
                          -outfile => 'query.bls',
                          -method_args => [ -html => ' ' );

• To get the name of the blast output file, do

  $file = $fac->blast_out;

• To clean up the temp files (you must do this explicitly):

  $fac->cleanup;

bl2seq()

Running bl2seq is similar, but both -query and -subject are required, and the attached database is ignored. The blast method must be specified explicitly with the -method parameter:

$fac->bl2seq( -method => 'blastp',
              -query => $seq_object_1,
              -subject => $seq_object_2 );

Other parameters ( -method_args, -outfile, and -outformat ) are valid.

Return values

The return value is always a Bio::Search::Result::BlastResult object on success, undef on failure.

SEE ALSO

Bio::Tools::Run::StandAloneBlastPlus, Bio::Tools::Run::BlastPlus

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

bioperl-l@bioperl.org                  - General discussion
http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

http://redmine.open-bio.org/projects/bioperl/

AUTHOR - Mark A. Jensen

Email maj -at- fortinbras -dot- us

Describe contact details here

CONTRIBUTORS

Additional contributors names and emails here

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

run()

Title   : run
Usage   :
Function: Query the attached database using a specified blast
          method
Returns : Bio::Search::Result::BlastResult object
Args    : key => value:
          -method => $method [blastp|blastn|blastx|tblastx|tblastn|
                              rpsblast|psiblast|rpstblastn]
          -query => $query_sequences (a fasta file name or BioPerl sequence
                     object or sequence collection object)
          -outfile => $blast_report_file (optional: default creates a tempfile)
          -outformat => $format_code (integer in [0..10], see blast+ docs)
          -method_args => [ -key1 => $value1, ... ] (additional arguments
                        for the given method)

bl2seq()

Title   : bl2seq
Usage   :
Function: emulate bl2seq using blast+ programs
Returns : Bio::Search::Result::BlastResult object
Args    : key => value
          -method => $blast_method [blastn|blastp|blastx|
                                    tblastn|tblastx]
          -query => $query (fasta file or BioPerl sequence object
          -subject => $subject (fasta file or BioPerl sequence object)
          -outfile => $blast_report_file
          -method_args => [ $key1 => $value1, ... ] (additional method
                       parameters)

next_result()

Title   : next_result
Usage   : $result = $fac->next_result;
Function: get the next BLAST result
Returns : Bio::Search::Result::BlastResult object
Args    : none

rewind_results()

Title   : rewind_results
Usage   : $fac->rewind_results;
Function: rewind BLAST results
Returns : true on success
Args    :

blast_out()

Title   : blast_out
Usage   : $file = $fac->blast_out
Function: get the filename of the blast report file
Returns : scalar string
Args    : none

cpanm Bio::DB::ESoap

perl -MCPAN -e shell
install Bio::DB::ESoap