/ 
Bio-GeneDesign-5.56
River stage zero No dependents
/ GD_Reverse_Translate.pl

NAME

GD_Reverse_Translate.pl

VERSION

Version 5.56

DESCRIPTION

Given at least one protein sequence as input, the Reverse_Translate script
generates synonymous nucleotide sequences using either a user-defined RSCU
table or an RSCU table provided by GeneDesign

If no algorithm is specified, the balanced algorithm will be used. These are
the algorithms provided by default with GeneDesign; you can make your own; see
developer docs.

Output will be named according to the name of the input file, and will be
tagged with the suffix _RT.

Algorithms:
  high: The high algorithm replaces every codon in the input sequence with
      the most translationally optimal codon as specified by the input RSCU
      tables or known RSCU tables (if organism is specified). If the codon is
      already the ideal codon it is left alone.
  balanced: The balanced algorithm uses the rscu data to determine a
       likelihood of codon replacement.

USAGE

-r OR -o must be provided. If both are given the table will be treated as
    another organism, named after the table's filename.

ARGUMENTS

Required arguments:

-i,   --input : a file containing protein sequences.
-org, --organism : an organism whose RSCU table can be found in the config
  directory, or several separated by commas
AND/OR
-r,   --rscu : path to an RSCU table generated by GD_Generate_RSCU_Table.pl,
  or several separated by commas
-r OR -o must be provided. If both are given the table will be treated as
    another organism, named after the table's filename.

Optional arguments:

-a,   --algorithm : protocol for replacing peptides; defaults to "balanced"
-out, --output : path to an output directory; defaults to GeneDesign's default
-f,   --format : default genbank
-s,   --split : output all sequences as separate files
-h,   --help : Display this message

COPYRIGHT AND LICENSE

Copyright (c) 2015, Sarah Richardson All rights reserved.

Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met:

* Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer.

* Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution.

* The names of Johns Hopkins, the Joint Genome Institute, the Lawrence Berkeley National Laboratory, the Department of Energy, and the GeneDesign developers may not be used to endorse or promote products derived from this software without specific prior written permission.

THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE DEVELOPERS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.